Services

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The Keck Facility provides a wide range of services for both the Whitehead/MIT community as well as the broader biomedical research community.

Light microscopy

Consultation

To maximize your success, the first step for use of the Keck Facility is a brief consultation with Cassandra Rogers to discuss your project and experimental goals as well as the resources available in the Keck. This will help us both determine the best fit and ensure the first experiment is as successful as possible.

Training

We are happy to train you on as many microscopes as your research requires. Training is scheduled for when you have your own samples ready to be imaged. Training includes an introduction to the equipment and software as well as basic guidelines for selecting settings. Advanced techniques, such as FRAP and large volume imaging, typically require multiple training sessions to reach independence. Refresher courses and advanced training are available for users previously trained.

Advice

To ensure you get the most out of microscopy we are available to discuss any and all aspects of your imaging - conceptual approaches, sample preparation, reagent purchases, image acquisition, troubleshooting and image processing and analysis. No question is too big or small; please contact us with any queries you have.

Imaging service

Limited imaging assistance on the light microscope may be available, schedule permitting.

Image analysis

Post-acquisition image processing and analysis is an important part of modern microscopy. We can provide advice and modest assistance for image analysis with Fiji/ImageJ, Imaris or Vision4D. We hold a service contract with Bitplane for assistance with advnced use of Imaris.  Whitehead researchers: Assistance with CellProfiler is available through a contract agreement--see related BARC page for details.

Help with lab equipment

If you need help with your lab microscope we might be able to assist (e.g. train you how to change the bulb). 

Education

Understanding how your tool works helps make your more adept at wielding it's power.  Please see our Education & Resources page for information on seminars, courses and online resources to learn more about microscopes, camera, probes, image analysis and more.

 

Electron Microscopy

As of August 22, 2019, the Keck Facility no longer provides direct service, support or training for the electron microscopy equipment. The equipment will remain on-site for already trained users.  Training for the Tecnai Spirit TEM is provided through the Peterson Nanotechnoogy Materials Core.