Zeiss Lightsheet Z.1
Image
Hardware
- Zeiss Lightsheet Z.1 light sheet fluorescence microscope
- 2 PCO.edge sCMOS cameras
- Solid state excitation lasers:
405nm, 440nm, 491nm, 514nm, 561nm, and 640nm - Transmitted light
- Zeiss Zen acquisition software
- 32TB temporary file storage
References:
- Required Consumables
- Sample mounting
- White paper (pdf)
- Video: Small samples (Capilary-based),
- Video: Examples/Ideas for Large samples
- Lightpath (pdf)
Filters:
Not sure which fluorophores will work best? Check out an online spectra viewer (see Links) or contact Cassandra.
| Pos | Menu name | Fluorophores | Camera 1 Filter | Camera 2 Filter |
| 1 | mirror | -- | -- | -- |
| 2 | LP490 | DAPI + GFP | 420-470nm | 505-545nm |
| 3 | LP510 | CFP + YFP | 460-500nm | 525-565nm |
| 4 | LP560 | GFP + mCherry | 505-545nm | LP 585nm |
| 5 | LP560 | DAPI & GFP + mCherry & DRAQ5 |
425-460nm & 505-545nm |
570-610nm & 670-735nm |
| 6 | LP640 | mCherry + DRAQ5 | 575-615nm | LP 660nm |
Objectives:
| Mag. | NA |
Immersion Medium |
RI |
|---|---|---|---|
| 5X | 0.15 | Air | 1.33 or 1.45 |
| 20X | 1.0 | Water dipping | 1.33 |
| 40X | 1.0 | Water dipping | 1.33 |
| 20X | 1.0 | CLARITY dipping | 1.45 |
Software:
- Zeiss Zen - basic post processing, data handling, manual 3D multiview reconstruction
- arivis vision4D - visualization of very large datasets
- Fiji - post processing, data handling, automated 3D multiview reconstruction