Zeiss LSM 710 Laser Scanning Confocal
- Zeiss AxioObserver motorized inverted microscope stand with DIC optics
- Motorized XY stage
- Zeiss LSM 710 laser scanning confocal with spectral imaging
- 3 PMT detectors (no spectral detector)
- 6 visible laser lines: Argon Gas laser for
458nm, 488nm, 514nm; solid state 405nm and 561nm; HeNe 594nm and 633nm - Coherent Chameleon Ultra II femtosecond pulsed-IR tunable from 690-1064nm for multiphoton imaging
- Transmitted light detector
- Zeiss Zen Black 2012 acquisition software
Filters:
Not sure which fluorophores will work best? Check out an online spectra viewer (see Links) or contact Cassandra.
Widefield (viewing samples by eye):
- DAPI
- FITC/GFP/Alexa 488
- Rhodamine/ Alexa 568
- DIC
Confocal emission is spread into a spectrum with a diffraciton grating. Any range of wavelengths 371-740nm can be sent to one of three PMT detectors. Note that PMT 3 has higher sensitivity for red wavelengths than the other two detectors. For specific information on thel asers and detection wavelengths used for an image, look in the metadata of your .lsm or .czi file.
Objectives:
| Mag. | NA |
Immersion Medium |
DIC | Corrections | Working Distance (mm) |
2 photon optimized? |
|---|---|---|---|---|---|---|
| 10X | 0.30 | Air | -- | Plan NeoFluar | 5.5 | |
| 25X | 0.8 corr | Water, Glycerol, or Oil | -- | LD LCI Plan Apochromat | 0.57 | YES |
| 40X | 1.1 corr | Water | III | LD C-Plan Apochromat | 0.62 | YES |
| 40x | 1.3 | Oil | III | Plan Apochromat | 0.21 | |
| 63X | 1.4 | Oil | III | Plan Apochromat | 0.19 |
corr = objective has a correction collar that must be adjusted to prevent spherical aberration.
Spatial scaling is dependent on the user-defined frame size and zoom. See your.lsm or .czi file.
Sample holders:
All objectives expect a #1.5 glass coverslip.
| Stage plates | Compatible Sample holders |
|---|---|
| Adjustable | Slides, LabTek II chambers, 35mm dishes, 60mm dishes |
| Multipwell plate | Any standard 96-well plate format |
Software:
To view your .lsm files in lab, see our Links page for Zen lite 2011 (requires Windows operating system) or the Fiji package of ImageJ.