Zeiss LSM 710 Laser Scanning Confocal

Hardware

Zeiss AxioObserver motorized inverted microscope stand with DIC optics
Motorized XY stage
Zeiss LSM 710 laser scanning confocal with spectral imaging
3 PMT detectors (no spectral detector)
6 visible laser lines: Argon Gas laser for
458nm, 488nm, 514nm; solid state 405nm and 561nm; HeNe 594nm and 633nm
Coherent Chameleon Ultra II femtosecond pulsed-IR tunable from 690-1064nm for multiphoton imaging
Transmitted light detector
Zeiss Zen Black 2012 acquisition software

Filters:

Not sure which fluorophores will work best? Check out an online spectra viewer (see Links) or contact Cassandra.

Widefield (viewing samples by eye):

DAPI
FITC/GFP/Alexa 488
Rhodamine/ Alexa 568
DIC

Confocal emission is spread into a spectrum with a diffraciton grating. Any range of wavelengths 371-740nm can be sent to one of three PMT detectors. Note that PMT 3 has higher sensitivity for red wavelengths than the other two detectors. For specific information on thel asers and detection wavelengths used for an image, look in the metadata of your .lsm or .czi file.

Objectives:

Mag.	NA	Immersion Medium	DIC	Corrections	Working Distance (mm)	2 photon optimized?
10X	0.30	Air	--	Plan NeoFluar	5.5
25X	0.8 corr	Water, Glycerol, or Oil	--	LD LCI Plan Apochromat	0.57	YES
40X	1.1 corr	Water	III	LD C-Plan Apochromat	0.62	YES
40x	1.3	Oil	III	Plan Apochromat	0.21
63X	1.4	Oil	III	Plan Apochromat	0.19

corr = objective has a correction collar that must be adjusted to prevent spherical aberration.
Spatial scaling is dependent on the user-defined frame size and zoom. See your.lsm or .czi file.

Sample holders:

All objectives expect a #1.5 glass coverslip.

Stage plates	Compatible Sample holders
Adjustable	Slides, LabTek II chambers, 35mm dishes, 60mm dishes
Multipwell plate	Any standard 96-well plate format

Software:

To view your .lsm files in lab, see our Links page for Zen lite 2011 (requires Windows operating system) or the Fiji package of ImageJ.