Zeiss LSM 700 confocal
Hardware
- Zeiss AxioObserver motorized inverted microscope stand
- Zeiss LSM 700 laser scanning confocal
- Solid state excitation lasers:
405nm, 491nm, 555nm, and 649nm - Transmitted light detector
- Interference relection imaging with 405nm
- Zeiss Zen 2010 acquisition software
Filters:
Not sure which fluorophores will work best? Check out an online spectra viewer (see Links) or contact Cassandra.
Widefield filter sets for viewing samples by eye:
- DAPI
- FITC/GFP
- Rhodamine
Confocal emission filters, used in conjunction with the variable secondary dichroic (VSD) beamsplitter
| Detector 1 | Detector 2 |
|---|---|
|
490-555nm |
505-600nm |
| 490-635nm | LP 490nm |
| SP 490nm | LP 560nm |
| SP 555nm | LP 615nm |
| SP 640nm | LP 640nm |
| 465-505nm + LP 525nm | 555-610nm + LP 650nm |
| 420-470nm + 500-610nm | 495-535nm + LP 555nm |
Objectives:
| Mag. | NA |
Immersion Medium |
DIC | Correction | Working Distance (mm) |
|---|---|---|---|---|---|
| 5X | 0.13 | NA | |||
| 10X | 0.45 | Air | NA | Plan NeoFluar | 2.0 |
| 20X | 0.8 | Air | II | Plan Apochromat | 0.55 |
| 25X | 0.8 | Water, Glycerol, or Oil | III | LCI Plan NeoFluar Corr | 0.21 |
| 40x | 1.3 | Oil | NA | Plan Apochromat | 0.21 |
| 63X | 1.4 | Oil | II | Plan Apochromat | 0.19 |
Software:
To view your .lsm files in lab, see our Links page for Zen lite 2011 (requires Windows operating system) or ImageJ.