Leica Stellaris 8 FALCON

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A group of colorful microglia with protrustions stretching towads each other
Hardware
  • Leica DMI8 Inverted microscope with Adaptive Focus Control
  • Leica Stellaris 8 FALCON DIVE AOBS
  • White Light Laser (FLIM)
    • tunable from 450-790nm
  • Solid State 405nm laser
  • Coherent Chameleon two-photon laser (FLIM)
    • tunable from 735-1035
  • Variable emission collection
  • OKO lab full enclosure incubation
  • LASX acquisition software

Filters:

Not sure which fluorophores will work best? Contact us!

Widefield filter sets for viewing samples by eye:

  • DAPI
  • FITC/GFP
  • Rhodamine

Confocal: Variable emission collection on 4 HyD detectors (2 HyD S and 2 HyD X

 

 

Objectives:

 

Mag.NAImmersion MediumCorrectionWorking
Distance (mm)
10X0.40AirPlan Apo2.0
20X0.75AirPlan Apo1
40X1.25GlycerolPlan Apo0.21
40x1.1WaterPlan Apochromat0.21
63X1.4OilPlan Apochromat0.19

 

Software:

To view your .lsm files in lab, see our Links page for Zen lite 2011 (requires Windows operating system) or ImageJ.