Abbelight SAFe 360 Nanoscopy System

Hardware

Abbelight SAFe MN360 super-resolution microscope
Evident IX83 inverted microscope frame with ZDC focus control System
Marzhauser SCANplus IM with Tango controler
4 laser lines in Abbelight Nexus Laser Combiner:
405nm – 50mW, 488nm – 40mW, 561nm – 300mW, and 640nm – 1000mW
2 Hamamatsu Orca Fusion camera
Abbelight Neo LiveImaging Software
Abbelight Neo Analysis Software

Supported imaging modalities:

Single molecule localization (dSTORM, PALM, and PAINT) and TIRF microscopy

Filter wheel configuration:

The system has filter wheels in front of each camera, Camera T correspond to the Transmitted camera, Camera R corresponds to the Reflected camera.

Position	Camera T	Camera R
1	Cy5	Cy5
2	Cy3	Cy3
3	GFP	GFP
4	DAPI	DAPI
5	4 Bands Filter (Cy5,Cy3,GFP,DAPI)	4 Bands Filter (Cy5,Cy3,GFP,DAPI)
6	Empty	Empty

Abbelight Simultaneous Multicolor Dichroic :

Abbelight instruments are designed to accommodate interchangeable dichroic cubes that split emission signals between two cameras, enabling simultaneous multi-color imaging. The available dichroic cubes are listed below:

Applications	Emission Dichroic Cube	*Camera T adapted fluorophores**	*Camera R adapted fluorophores**
SMLM or TIRF	560LP	Cy5, Cy3	DAPI, GFP
SMLM or TIRF	640 LP	Cy5	Cy3, DAPI, GFP
SMLM	700LP Spectral-demixing	AF647, CF660, CF680	AF647,CF660,CF680

Objectives:

Magnification	Numerical Aperture	Immersion Medium	Corrections	**Scaling (um/pixel)
20x	0.75	Air/Dry	UPlanSApo	0.485
100x	1.5	Oil	UPLAPO	0.097

Sample holder:

Marzhauser - Universal stage insert IM

Software:

Abbelight Neo LiveImaging :

Multi-Dimensional Acquisition, 2D and 3D Live detection and reconstruction, ROI toolbox, Live statistics, Automatic calibration tools for illumination, multichannel alignment, smart notebook

Abbelight NEO analysis:

Batch localization, 3D viewer, Multicolor channel alignment, Filtering, 2D and 3D Clustering, 2D and 3D Colocalization, SPT analysis, Multicolor analysis: sequential and automatic spectral demixing.