Zeiss AxioPlan 2
Filters:
Not sure which fluorophores will work best? Use an online spectra viewer (see Links) or contact Cassandra.
| Software setting | Excitation | Dichroic | Emission | Source |
|---|---|---|---|---|
| DAPI | G 365 | 445/50M | Zeiss (filter 49) | |
| GFP | HQ470/40X | HQ525/50M | Chroma | |
| TxRed | 565/40X | FT585 | 620/60x | Zeiss (filter 31) |
| Cy5 | 628/40X | 692/40M | Chroma | |
| CFP | D436/20x | D480/40m | Chroma | |
| YFP | D500/20x | D535/30m | Chroma | |
| DIC | Tungsten Halogen | Analyzer | Zeiss | |
| Trans | Tungsten Halogen | -- |
All numbers in nanometers.
Objectives:
| Mag. | NA |
Immersion Medium |
Phase contrast | DIC prism | Corrections | HamamatsuScaling** (um/pixel) |
|---|---|---|---|---|---|---|
| 5x | 0.25 | Air | -- | -- | Fluar | |
| 10x | 0.30 | Air | -- | I | EC Plan NeoFluar | 0.645 |
| 20x | 0.5 | Air | Ph1 | II | Plan NeoFluar | 0.317 |
| 40x | 0.75 | Air | Ph2 | II | Plan Fluor | 0.162 |
| *40x | 1.3 | Oil | -- | II | Plan NeoFluar | 0.163 |
| 63x | 1.4 | Oil | -- | III | Plan Apochromat | 0.102 |
| 100x | 1.4 | Oil | Ph3 | III | Plan Apochromat | 0.065 |
| *100x | 1.4 | Oil | -- | III | Plan Apochromat | 0.065 |
*Objectives marked with an astrisk are not permanently installed. Please contact Wendy for installation.
** These values work well for making scale bars. When performing careful distance measurements, it is best practice to confirm the calibration for the exact setup you use.
Sample holders:
Standard 3 x 1in slides with #1.5 glass coverslip
Software:
To view files in lab, many users like to use Fiji (ImageJ) with the BioFormats plugin for importing the .nd file. See our Links page to download.