Nikon TE2000 Long Term Live Cell Imaging
- Nikon TE2000 inverted microscope stand with DIC and phase contrast optics
- Prior Proscan II motorized ZY stage and focus motor
- Uniblitz fluorescence and transmitted light shutters
- EXFO metal halide light source
- Solent Scientific incubation enclosure
- Hamamatsu Orca-ER cooled CCD camera
- MetaMorph aquisition software
Filters:
Not sure which fluorophores will work best? Use an online spectra viewer (see Links) or contact Wendy.
| Software setting | Excitation | Dichroic | Emission |
|---|---|---|---|
| DAPI | D360/40X | ET460/50M | |
| GFP | HQ480/40X | 535/50M | |
| RFP | 560/40X | D630/60M | |
| Cy5 | 628/40X | 692/40M | |
| DIC | Tungsten halogen | Analyzer | |
| Brightfield | Tungsten halogen | Specified filtercube | |
| *CFP | ET436/20x | T455lp | ET480/40m |
| YFP | ET500/20x | T515lp | ET535/30m |
| *CFP/YFP FRET | ET436/20x | T455lp | ET535/30m |
| *BFP | ET405/20x | T425lpxr | ET460/50m |
All numbers in nanometers.
*Filters marked with an astrisk are not permanently installed. Contact Wendy for use of these.
Objectives:
| Mag. | NA |
Immersion Medium |
Phase contrast | DIC | Corrections | **Scaling (um/pixel) |
|---|---|---|---|---|---|---|
| 10x | 0.30 | Air | Ph 1 | -- | Plan Fluor | 0.647 |
| 20x ELWD | 0.45 corr | Air | Ph1 | -- | Plan Fluor | 0.328 |
| 40x ELWD | 0.6 corr | Air | Ph2 | -- | Plan Fluor | 0.166 |
| *40x | 0.95 | Air | -- | H | Plan Apo | 0.164 |
| *40x | 1.3 | Oil | -- | H | Plan Apo | |
| *60x | 1.4 | Oil | *Ph3 | -- | Plan Apo | 0.108 |
| *100x | 1.4 | Oil | *Ph3 | -- | Plan Apo |
corr = objective has a correction collar that must be adjusted to prevent spherical aberration.
*Objectives marked with an astrisk are not permanently installed. Please contact Wendy for installation.
** These values work well for making scale bars. When performing careful distance measurements, it is best practice to confirm the calibration for the exact setup you use.
Sample holders:
All objectives, except ELWD, expect a #1.5 glass coverslip. ELWD can work with either glass coverslips or plastic dishes. Glass must be used when imaging fluorescence.
Stage platesCompatible Sample holders
AdjustableSlides, LabTek II chambers, 35mm dishes, 60mm dishes
Multipwell plateAny standard 96-well plate format
Software:
To view files in lab, many users like to use Fiji (ImageJ) with the BioFormats plugin for importing the .nd file. See our Links page to download.