DeltaVision Elite Widefield Deconvolution

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GE HC DeltaVision Elite microscope
Hardware

Our DeltaVision Elite Imaging System includes:

  • Olympus iX81 stand with GEHC modifications
  • Motorized XYZ stage
  • Perfect Focus laser-based focus stabilization
  • Solid-state fluorescence illumination with fast filter wheel
  • Incubation enclosure
  • PCO-edge sCMOS camera
  • SoftWorx acquisition software

Filters:

Not sure which fluorophores will work best? Use an online spectra viewer (see Links) or contact Cassandra.

This system has three multiband polychroic mirrors paired with excitation and emission filters that are housed in filterwheels. The polychromatic mirros are:

  • DAPI / FITC / TRITC / Cy5 = "D/F/TRITC/Cy5"
  • DAPI / FITC (or GFP)/ mCherry / Cy5 = "D/F/mCherry/Cy5"
  • CFP / YFP / mCherry = "C/Y/mCh"
Software setting Excitation Dichroic Emission
DAPI 390/18nm D/F/TRITC/Cy5 or
D/F/mCherry/Cy5		 435/48nm
GFP/FITC 475/28nm D/F/TRITC/Cy5 or
D/F/mCherry/Cy5		 525/48nm
TRITC 542/27nm D/F/TRITC/Cy5 597/45nm
mCherry 575/25nm D/F/mCherry/Cy5 or C/Y/mCh 625/45nm
Cy5 632/22nm D/F/TRITC/Cy5 or
D/F/mCherry/Cy5		 679/34nm
POL White LED na Analyzer
YFP 438/24nm C/Y/mCh 475/24nm
CFP 513/17nm C/Y/mCh 548/22nm

 

Objectives:

Mag. NA

Immersion Medium

 DIC Corrections **Scaling (um/pixel)
10x 0.40 Air -- Plan Fluor 0.668
20x 0.75 Air -- UPlanSApo 0.331
60x 1.42 Oil -- PlanApo N 0.108
100x 1.4 Oil -- UPlanSApo 0.065

** These values work well for making scale bars. When performing careful distance measurements, it is best practice to confirm the calibration for the exact setup you use.

 

Helpful Documents:

Sample holders:

All objectives expect a #1.5 glass coverslip.

Stage plates Compatible Sample holders
Repeatable multi-insert stage Slides, LabTek II chambers, 35mm dishes
Stage ring Other formats, including portions of multiwell plates

 

Software:

To view files in lab, many users like to use Fiji (ImageJ) with the BioFormats plugin for importing the .dv file. See our Links page to download.