Nikon Ti2 Screening Microscope
Image
Hardware
Our Ti2 Screening Microscope includes:
- Nikon Ti2 inverted microscope
- Motorized XYZ stage
- Perfect Focus laser-based focus stabilization
- Celesta II 7 line laser light source with fast filter wheel
- Incubation enclosure
- Photometrix Kenetix large format sCMOS camera
- Nikon Elements with JOBS acquisition software
Filters:
Not sure which fluorophores will work best? Use an online spectra viewer (see Links) or contact Cassandra.
| Software setting | Excitation | Dichroic | Emission |
|---|---|---|---|
| DAPI | 390/18nm | D/F/TRITC/Cy5 or D/F/mCherry/Cy5 |
435/48nm |
| GFP/FITC | 475/28nm | D/F/TRITC/Cy5 or D/F/mCherry/Cy5 |
525/48nm |
| TRITC | 542/27nm | D/F/TRITC/Cy5 | 597/45nm |
| mCherry | 575/25nm | D/F/mCherry/Cy5 or C/Y/mCh | 625/45nm |
| Cy5 | 632/22nm | D/F/TRITC/Cy5 or D/F/mCherry/Cy5 |
679/34nm |
| POL | White LED | na | Analyzer |
| YFP | 438/24nm | C/Y/mCh | 475/24nm |
| CFP | 513/17nm | C/Y/mCh | 548/22nm |
Objectives:
| Mag. | NA |
Immersion Medium |
DIC | Corrections | **Scaling (um/pixel) |
|---|---|---|---|---|---|
| 10x | 0.40 | Air | -- | Plan Fluor | 0.668 |
| 20x | 0.75 | Air | -- | UPlanSApo | 0.331 |
| 60x | 1.42 | Oil | -- | PlanApo N | 0.108 |
| 100x | 1.4 | Oil | -- | UPlanSApo | 0.065 |
** These values work well for making scale bars. When performing careful distance measurements, it is best practice to confirm the calibration for the exact setup you use.
Sample holders:
| Stage plates | Compatible Sample holders |
|---|---|
| 35mm dish/Slide insert | Slides, LabTek II chambers, 35mm dishes |
| Multi-well plate insert | All Multi-well plates |
Software:
Nikon Elements with JOBS